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Lesson 1.9 · AQA Combined Science

Osmosis

Water crosses a membrane

Osmosis is the net movement of water from a dilute solution to a more concentrated solution through a partially permeable membrane.

Dilute means less dissolved solute and a higher concentration of water. Concentrated means more dissolved solute and a lower concentration of water.

Water molecules move both ways, but there is net movement towards the concentrated solution. No energy from respiration is needed.

Predict what happens to plant tissue

In a more dilute solution

Water enters by osmosis. Potato tissue gains mass. Plant cells become firm (turgid) as water fills their vacuoles.

In a more concentrated solution

Water leaves by osmosis. Potato tissue loses mass. Plant cells lose firmness.

Required practical · potato cylinders

Investigate how sugar or salt concentration affects the mass of plant tissue.

  1. Prepare a range of solution concentrations. Cut potato cylinders of equal length and diameter.
  2. Gently blot each cylinder and record its initial mass.
  3. Place cylinders in the solutions. Keep solution volume, temperature and immersion time the same.
  4. Remove the cylinders after the set time. Gently blot off surface solution and record final masses.
  5. Calculate percentage changes. Repeat each concentration with several cylinders and calculate a mean.

Keep the potato source consistent. Use cutting tools as your teacher directs.

Independent variable: solution concentration. Dependent variable: percentage change in mass.

Calculate percentage mass change

Percentage change = final mass − initial massinitial mass × 100

Example: 5.0 g becomes 5.5 g. Change = +0.5 g.

0.55.0 × 100 = +10%

Positive means a gain; negative means a loss. Percentages allow fairer comparisons when starting masses differ.

Read the graph

Put solution concentration on the x-axis and mean percentage mass change on the y-axis.

Where the graph crosses 0% change, there is no net movement of water. This estimates the concentration that balances the potato tissue.

Example results, not measured class data. The zero crossing is 0.4 mol/dm³.

Challenge · rate of uptake

Average rate of water uptake can be estimated from mass gain over time.

Rate of mass gain = mass gainedtime

If a cylinder gains 0.6 g in 30 minutes, the average rate is 0.02 g/min.

Remember

Water moves from dilute to concentrated solution through a partially permeable membrane. Mass gain means water entered; mass loss means water left.